Abstract. A new azasterol 22-hydrazine-imidazolin-2-yl-chol-5-ene-3β-ol (AH3) and their derivatives [Au(AH3)Cl] (1) and [Cu(AH3)2(H2O)2](NO3)2 (2) were synthesized and characterized by a combination of elemental analyses, 1H and 13C -NMR, UV-vis and infrared spectroscopies. The interactions with two targets DNA and TrxR were investigated. The results from these studies suggested that these metal-azasterol complexes interact with DNA most probably by covalent binding. Complex 1 showed also the ability to strongly inhibit TrxR. Additionally, their activities against yeast cells of Sporothrix spp. were evaluated; finding that complex 1 was the most active, even better than itraconazole.

Resumen. Un nuevo azasterol 22-hidrazina-imidazolin-2-il-col-5-en-3β-ol (AH3) y sus derivados [Au(AH3)Cl] (1) y [Cu(AH3)2(H2O)2](NO3)2 (2) fueron sintetizados y caracterizados por técnicas espectroscopias como RMN 1H ,13C, UV-vis, infrarrojo y análisis elemental. Se investigaron las interacciones de estos compuestos sobre dos blancos moleculares el ADN y la TrxR. Los resultados encontrados sugirieren que estos complejos metal-azasterol interactúan con el ADN covalentemente. El complejo 1 también mostró tener la capacidad de inhibir fuertemente la TrxR. Además, fueron evaluadas sus actividades contra Sporothrix spp., encontrando que complejo 1 fue el más activo, incluso mejor que el itraconazol.

RESUMO Nesta pesquisa realizou-se a caracterização pluvi-fluviométrica da Bacia do Rio das Fêmeas em São Desidério (BA) e avaliaram-se as modificações das vazões, considerando a presença de uma Pequena Central Hidrelétrica (PCH) e as alterações na cobertura do solo. Selecionaram-se as estações da rede da Agência Nacional de Águas (ANA) para análise dos dados históricos de vazão e precipitação (1985-2013); utilizaram-se imagens dos satélites Landsat 5 e IRS-P6 (RESOURCESAT-1) dos anos 1985, 1995, 2000, 2005, 2008, 2011 e 2012 para classificação supervisionada da cobertura do solo em duas classes distintas: áreas com vegetação natural e áreas com interferências antrópicas, cujas regiões possuíam algum tipo de uso. Além disso, foram avaliadas as alterações das vazões medianas e médias anuais antes e após a instalação da PCH. Os resultados deste trabalho mostraram que os dados anuais médios de vazão, ao longo do tempo, reduziram com uma magnitude maior que os dados médios anuais de precipitação na bacia estudada. Em relação à ocupação do solo, a área antropizada aumentou ao longo do tempo. Diante deste estudo verificou-se que a presença do reservatório da PCH possibilitou redução nos picos de vazões máximas e aumento nos valores baixos das vazões mínimas. No período pós-PCH ocorreram vazões médias e medianas inferiores ao período pré-PCH. Entretanto, estes resultados foram influenciados pela combinação do aumento das atividades antrópicas com a variação da precipitação média anual na bacia.

ABSTRACT In this research it was studied a pluvi-fluviometric characterization and the flow rates changes of Fêmeas River basin located in São Desidério (BA), considering the presence of a PCH and the cover soil alterations. Therefore the network stations of the Agência Nacional de Águas were selected to analyze the rainfalls and flows historical data (from 1985 to 2013); LandSat 5 and IRS-P6 (RESOURCESAT-1) satellite images of 1985, 1995, 2000, 2005, 2008, 2011 and 2012 years were used for land cover classification in two classes: natural; and areas with anthropic influence, in whose regions there was some kind of use; and the changes in the median and average annual flows before and after the PCH installation were evaluated. The results of this research showed that the annual average flow data over the study period reduced in a magnitude higher than the annual average rainfall data. In relation to the soil land use, the anthropic area increased over time. With this study it was found that the presence of the PCH reservoir allowed a reduction in the maximum and minimum flow rates peak. It was observed that in the post-PCH period both average and median flow rate data were lower than that those measured in the pre-PCH period. Nevertheless, these results were influenced by the combination of the human activities that increased over time and the annual average rainfall data variation in the basin.

Sporotrichosis is the most frequent subcutaneous mycosis in the world and its increasing incidence has led to the search for new therapeutic options for its treatment. In this study, we demonstrated that three structural analogues of miltefosine (TCAN26, TC19, and TC70) showed inhibitory activity against Sporothrix schenckii sensu stricto and that TCAN26 was more active in vitro than miltefosine against several isolates. Scanning electron microscopy showed that S. schenckii exposure to TCAN26 resulted in cells that were slightly more elongated than untreated cells. Transmission electron microscopy showed that TCAN26 treatment induced loss of the regular cytoplasmic electron-density and altered the cell envelope (disruption of the cell membrane and cell wall, and increased cell wall thickness). Additionally, TCAN26 concentrations required to kill S. schenckii cells were lower than concentrations that were cytotoxic in mammalian cells, and TCAN26 was more selective than miltefosine. Thus, the adamantylidene-substituted alkylphosphocholine TCAN26 is a promising molecule for the development of novel antifungal compounds, although further investigations are required to elucidate the mode of action of TCAN26 in S. schenckii cells.

Toxoplasma gondii causes toxoplasmosis, a worldwide disease. Experimentation with pigs is necessary for the development of new therapeutic approaches to human diseases. BR-1 mini pigs were intramuscularly infected with T. gondii with tachyzoites (RH strain) or orally infected with cysts (ME-49 strain). Haematology and serum biochemistry were analysed and buffy coat cells were inoculated in mice to determine tachyzoite circulation. No alterations were observed in erythrocyte and platelet values; however, band neutrophils increased seven days after infection with ME-49. Serology of the mice inoculated with pig blood leucocytes revealed circulating ME-49 or RH strain tachyzoites in the pigs' peripheral blood at two and seven or nine days post-infection. The tachyzoites were also directly observed in blood smears from the infected pigs outside and inside leucocytes for longer periods. Alanine-aminotransferase was high at days 21 and 32 in the RH infected pigs. After 90 days, the pigs were euthanised and their tissue samples were processed and inoculated into mice. The mice serology revealed the presence of parasites in the hearts, ileums and mesenteric lymph nodes of the pigs. Additionally, cysts in the mice were only observed after pig heart tissue inoculation. The infected pigs presented similar human outcomes with relatively low pathogenicity and the BR-1 mini pig model infected with ME-49 is suitable to monitor experimental toxoplasmosis.

Trichuris trichiura is a soil-transmitted helminth which is prevalent in warm, moist, tropical and subtropical regions of the world with poor sanitation. Heavy whipworm can result either in Trichuris dysenteric syndrome - especially in children - or in a chronic colitis. In heavy infections, worms can spread proximally and may cause ileitis. Here we provide first microscopic evidence for a T. trichiura adult worm embedded in the rectum of a post-Colonial Brazilian adult mummy. During Colonial and post-Colonial times, many European chroniclers described a parasitic disease named Maculo whose symptomatology coincides with heavy helminthiasis. Based on our findings and on comparison of ancient textual evidence with modern description of heavy whipworm, we feel confident in considering that the two syndromes are expressions of the same pathological condition.

Phytomonas serpens are flagellates in the family Trypanosomatidae that parasitise the tomato plant (Solanum lycopersicum L.), which results in fruits with low commercial value. The tomato glycoalkaloid tomatine and its aglycone tomatidine inhibit the growth of P. serpens in axenic cultures. Tomatine, like many other saponins, induces permeabilisation of the cell membrane and a loss of cell content, including the cytosolic enzyme pyruvate kinase. In contrast, tomatidine does not cause permeabilisation of membranes, but instead provokes morphological changes, including vacuolisation. Phytomonas treated with tomatidine show an increased accumulation of labelled neutral lipids (BODYPY-palmitic), a notable decrease in the amount of C24-alkylated sterols and an increase in zymosterol content. These results are consistent with the inhibition of 24-sterol methyltransferase (SMT), which is an important enzyme that is responsible for the methylation of sterols at the 24 position. We propose that the main target of tomatidine is the sterols biosynthetic pathway, specifically, inhibition of the 24-SMT. Altogether, the results obtained in the present paper suggest a more general effect of alkaloids in trypanosomatids, which opens potential therapeutic possibilities for the treatment of the diseases caused by these pathogens.

Há décadas um escalpo peruano, datado do período pré-colombiano, foi examinado por um pesquisador da Fundação Oswaldo Cruz. O Professor Olympio da Fonseca Filho descreveu lêndeas e adultos fixos a fios de cabelos e fez comentários sobre a origem da infecção por piolhos na espécie humana. Este mesmo escalpo foi enviado ao nosso laboratório e é objeto deste artigo. Sua análise mostrou maciça infestação, com nove lêndeas/cm2 em impressionante número de adultos muito bem preservados. O tempo de infestação foi estimado em cerca de nove meses antes da morte, baseado na maior distância entre lêndeas e o couro cabeludo, levando em consideração taxa média de crescimento capilar de 1 cm por mês. Um pequeno pedaço de tecido tradicional peruano foi encontrado associado ao escalpo, provavelmente pertencente ao conjunto de peças usado no ritual funerário. Aqui, apresentamos alguns aspectos morfológicos de adultos e lêndeas vizualizados por microscopia eletrônica de varredura de baixo vácuo.

A pre-Columbian Peruvian scalp was examined decades ago by a researcher from the Oswaldo Cruz Foundation. Professor Olympio da Fonseca Filho described nits and adult lice attached to hair shafts and commented about the origin of head lice infestations on mankind. This same scalp was sent to our laboratory and is the subject of the present paper. Analysis showed a massive infestation with nine eggs/cm2 and an impressive number of very well preserved adult lice. The infestation age was roughly estimated as nine months before death based on the distance of nits from the hair root and the medium rate of hair growth. A small traditional textile was associated with the scalp, possibly part of the funerary belongings. Other morphological aspects visualized by low-vacuum scanning electron microscopy are also presented here for adults and nits.

A irrigação por gotejamento subsuperficial, que utiliza um sistema protetor do emissor para evitar entupimento do mesmo por raiz e partículas sólida do solo, pode ser viável em relação a um sistema convencional. Propôs-se, com este trabalho, avaliar o desempenho de um sistema para proteção de emissor e comparar os resultados com um sistema que utiliza emissor convencional para irrigação por gotejamento subsuperficial. O sistema com protetor foi construído com materiais de baixo custo: mangueira de polietileno, microtubo, conector e um gotejador para controlar a vazão e no sistema convencional utilizou-se um emissor comercial. Após 12 meses de avaliação o sistema com protetor demonstrou bom desempenho com vazão relativa média de 0,97 e 0,98 em vasos com e sem cultura, respectivamente, não apresentando problemas de entupimento e tendo menor custo. No sistema convencional constatou-se vazão relativa de 0,51 e 0,98 em vasos com e sem cultura, respectivamente, grau de entupimento por raiz de 49,22% e emissores com solo em seu interior. Desta forma, o uso do emissor com sistema de proteção indicou viabilidade para irrigação subsuperficial por gotejamento, nas condições desta pesquisa.

Subsurface drip irrigation that uses an emitter protection system to avoid its clogging by roots and soil particles may be viable compared to a conventional system. The objective of this work was to evaluate the performance of a system with emitter protection, and to compare the results with a system that uses a conventional emitter for subsurface drip irrigation. In the system with protection an inexpensive materials polyethylene hose, microtube, connector, and a dripper to control the flow rate were used; and, in the conventional system a commercial emitter was used. After 12 months of evaluation, the system with protector showed good performance, with relative average flow rate of 0.97 and 0.98 in pots with and without crop, respectively, showing no clogging problems and lower cost. In conventional system relative flow rate of 0.51 and 0.98 were observed in pots with and without crop, respectively, also clogging degree by roots of 49.22%, and emitters with soil inside was observed. Thus, the use of emitter with protection presented feasibility for subsurface drip irrigation, under conditions used in this research.

Este trabalho foi realizado com o objetivo de determinar as perdas de solo e água em um Argissolo Vermelho-Amarelo, submetido a quatro diferentes padrões de precipitação de chuva simulada e duas condições de cobertura: área com solo descoberto e com palhada após plantio de milho. O experimento foi conduzido no Campo Experimental da Embrapa-Agrobiologia, localizado no município de Seropédica-RJ, e consistiu na aplicação de chuvas simuladas com diferentes padrões, caracterizados como avançado (AV), intermediário (IN), atrasado (AT) e constante (CT), em uma área amostral de 0,80 m de largura por 1,0 m de comprimento. Utilizando um simulador de chuvas portátil, foram aplicadas chuvas com 30 mm de lâmina total durante 60 minutos. Nos padrões com intensidade variada, o pico de precipitação foi de 110 mm h-1. Os resultados obtidos possibilitaram concluir que a cobertura do solo com resíduos de milho reduziu substancialmente as taxas e perdas de água e solo, principalmente para o padrão de chuva constante, indicando que a palhada deixada sobre o solo após a colheita auxilia de maneira efetiva no controle da erosão.

This study was carried out in order to determine the soil and water losses in a Yellow-red Ultisol, under four different rainfall patterns and two conditions of soil cover: area with soil uncovered and with straw after corn planting. The experiment was conducted at the experimental field of Embrapa Agrobiologia, located at Seropédica municipality, State of Rio de Janeiro, Brazil. The treatments consisted in application of simulated rainfall using variable intensity classified in different patterns as: advanced (AV), intermediate (IN), delayed (AT), and constant (CT), in a plots with 0.8 m of width and 1.0 m of length. Using a portable rainfall simulator rainfall with 30 mm total depth during 60 minutes was applied. For the patterns with varied intensity, the peak of precipitation was 110 mm h-1. The results showed the cover of the soil with corn stubble substantially reduced the rates and the soil and water losses, mainly for the pattern of constant rainfall, indicating that the residues left on the ground after harvest helps effectively in the erosion control.

For many years, prokaryotic cells were distinguished from eukaryotic cells based on the simplicity of their cytoplasm, in which the presence of organelles and cytoskeletal structures had not been discovered. Based on current knowledge, this review describes the complex components of the prokaryotic cell cytoskeleton, including (i) tubulin homologues composed of FtsZ, BtuA, BtuB and several associated proteins, which play a fundamental role in cell division, (ii) actin-like homologues, such as MreB and Mb1, which are involved in controlling cell width and cell length, and (iii) intermediate filament homologues, including crescentin and CfpA, which localise on the concave side of a bacterium and along its inner curvature and associate with its membrane. Some prokaryotes exhibit specialised membrane-bound organelles in the cytoplasm, such as magnetosomes and acidocalcisomes, as well as protein complexes, such as carboxysomes. This review also examines recent data on the presence of nanotubes, which are structures that are well characterised in mammalian cells that allow direct contact and communication between cells.

O presente trabalho teve como objetivos, desenvolver dois modelos matemáticos para dimensionamento de microtubos em irrigação localizada, operando sob regime de escoamento turbulento, nos quais foram incluídas as perdas de carga no microtubo, perda de carga localizada e perdas por energia de velocidade na saída do microtubo. Para a determinação da perda de carga localizada, obteve-se, por meio de dados de laboratório, valores de coeficientes K de dissipação de energia na entrada do microtubo. Os modelos foram avaliados com o dimensionamento de uma linha lateral de irrigação e tiveram ótimos desempenhos, tendo em vista que os dados coletados proporcionaram vazão média próxima da vazão de projeto, com coeficiente de determinação acima de 98%, excelente uniformidade de distribuição, baixo erro médio absoluto e coeficiente de variação de vazão. Dessa forma, pode-se dizer que o sistema de irrigação com microtubos, em regime de escoamento turbulento, mostrou-se tecnicamente viável para utilização, e que os modelos matemáticos desenvolvidos foram adequados para a avaliação das perdas no sistema.

The present work was carried out in order to develop two mathematics models for design drip irrigation with microtubs, under turbulent flow. In the models were taken account the local head loss in the microtub, local head loss and head loss due to velocity energy in the microtub outside. To determine the local head loss it was calculated the K coefficients of the energy dissipation at the entrance of the microtub through laboratory tests. The models were evaluated through the design of an irrigation lateral line, ant it was obtained great performance with determination coefficient of 98%, the discharge observed were close to the projected discharge, excellent distribution uniformity, low mean absolute error and variation of discharge coefficient. According to the results, it can be conclude that the microtub drip irrigation operating under turbulent regime is technically feasible and the developed of mathematical models are adequate to evaluate the head loss in the system.

Visou-se, neste trabalho, determinar as perdas de solo, água e nutrientes em um Argissolo Vermelho-Amarelo submetido a quatro diferentes padrões de chuva simulada. O estudo foi conduzido no Campo Experimental da Embrapa-Agrobiologia, localizado no município de Seropédica, RJ, e consistiu na aplicação de chuvas simuladas com diferentes padrões caracterizados como avançado (AV), intermediário (IN), atrasado (AT) e constante (CT), em uma área amostral de 0,80 m de largura por 1,0 m de comprimento. As chuvas tiveram duração de 60 min com uma lâmina total aplicada de 30 mm e um pico de 110 mm h-1, para os padrões de intensidade variável. Os resultados obtidos possibilitaram concluir que após 60 min de chuva simulada as perdas de solo e água observadas para o padrão atrasado foram, em média, superiores em 61,6, 46,4 e 13,6%, respectivamente, para o CT, AV e IN (solo), e de 42,0, 19,0 e 19,0%, respectivamente, para o AV, IN e CT (água) e as perdas de nutrientes não diferiram entre os padrões de precipitação, sendo mais influenciadas pelas perdas de água escoada superficialmente que pelo material sólido em suspensão.

This study was carried out in order to determine the soil, water and nutrient losses in a Yellow-Red Ultisol, under four different patterns of simulated rainfall. The experiment was conducted at the Embrapa Agrobiologia experimental field, located in the municipality of Seropédica, Rio de Janeiro, Brazil. The study consisted in the application of simulated rains using variable rainfall intensity classified in different patterns as: advanced (AD), intermediate (IN), delayed (DE), and constant (CT), in plots 0.8 m wide and 1.0 m long. The rain had a duration of 60 min, with a total applied depth of 30 mm, and a peak of 110 mm h-1, for the variable intensity patterns. With the results obtained it was possible to verify that after 60 min of simulated DE pattern provided losses higher than CT, AD and IN, in 61.6, 46.4 and 13.6%, respectively (soil), and 42.0, 19.0 and 19.0% (run-off), respectively, for the AD, IN and CT patterns; and the nutrients losses did not differ among the rainfall patterns, being more affected by water run-off than solid material in suspension.

The association of the pellicle with cytoskeletal elements in Toxoplasma gondii allows this parasite to maintain its mechanical integrity and makes possible its gliding motility and cell invasion. The inner membrane complex (IMC) resembles the flattened membrane sacs observed in free-living protozoa and these sacs have been found to associate with cytoskeletal proteins such as articulins. We used immunofluorescence microscopy to characterise the presence and distribution of plateins, a sub-family of articulins, in T. gondii tachyzoites. A dispersed labelling of the whole protozoan body was observed. Electron microscopy of detergent-extracted cells revealed the presence of a network of 10 nm filaments distributed throughout the parasite. These filaments were labelled with anti-platein antibodies. Screening the sequenced T. gondii genome, we obtained the sequence of an IMC predicted protein with 25% identity and 42% similarity to the platein isoform alpha 1 present in Euplotes aediculatus, but with 42% identity and 55% similarity to that found in Euglena gracilis, suggesting strong resemblance to articulins.

Since the initial description of Trypanosoma cruzi by Carlos Chagas in 1909, several research groups have used different microscopic techniques to obtain detailed information about the various developmental stages found in the life cycle of this intracellular parasite. This review describes the present knowledge on the organization of the most important structures and organelles found in the protozoan, such as the cell surface, flagellum, cytoskeleton, kinetoplast-mitochondrion complex, glycosome, acidocalcisome, contractile vacuole, lipid inclusions, the secretory pathway, endocytic pathway and the nucleus.

Historically, scientists in Brazil has significantly contributed to the biology, cultivation and structural organization of the pathogenic protozoan Toxoplasma gondiiand its interaction with host cells, starting with the description of the protozoan by Splendore in 1908. The intracellular and extracellular corpuscoli observed in rabbits, corresponded to what we now as tachyzoites. Later on, a pioneering method to grow T. gondii in tissue cultures was developed by Guimarães and Meyer, 1942. They also observed for the first time T. gondii by transmission electron microscopy and made the initial description of the cytoskeleton of T. gondii by observing negatively stained cells. In the 1980's, the relation of the cytoskeleton with the sub-pellicular microtubules was reveled by freeze-fracture. More recently, several Brazilian groups have analyzed in detail basic aspects of the early interaction of the protozoan with the host cell, such as the role of protein phosphorylation, transfer of host cell surface components to the protozoan and genesis and organization of the parasitophorous vacuole. Tachyzoites strategically inhibit nitric oxide production during active invasion of activated macrophages. In vitro studies on the sexual cycle of T. gondii using primary cultures of cat enterocytes and the egress from host cells are being carried out. Perspectives are that the contribution of Brazilian science to the knowledge on T. gondii biology will continue to flourish in years to come.